Welcome to Hulab!

Welcome to Hulab!

Welcome to Hulab!

Welcome to Hulab!

Welcome to Hulab!





Immunogenomics Laboratory

ess

Genome Editing

Safety assessment of current genome editing toolboxes and development of new editing tools based on the mammalian immune system

Genome Integrity

To study how the DNA response and repair system deals with DNA breaks in the context of chromatin control to maintain the genome integrity of lymphocytes

Antibody Evolution

To develop sequencing-based screening systems to identify monoclonal antibodies and design a cell-based system for antibody generation, maturation, and evolution

Antibody Diversification

To study the regulatory mechanism of lymphocyte receptor diversity including RAG-mediated V(D)J recombination and AID-mediated class switch recombination and somatic hypermutation



service-image

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.

ess

service-image

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.

ess

service-image

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.

ess



Great Team

    Our laboratory is well-established for self-motivated young scientists. We warmly welcome you to join us and build a positive, innovative, and active young team together. Our lab will make every effort to create opportunities for each participant to develop their talents and realize their aspirations.


doctor

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.

read more

RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.

Education:

2006-2012      Ph.D        Peking University
2002-2006      B.S.          Peking University


Working Experiences:

2016-              PI           Peking University
2012-2016      Postdoc  Harvard Medical School


Awards:

2018        Qiu Shi Outstanding Youth Scholar
2016        Annual Distinguished Research Award in Life Science for Harvard Chinese
2014        Postdoctoral Fellowship at the American Cancer Research Center
2012        Ray Wu Prize



doctor

Dr. Yang Liu

read more

Dr. Yang Liu

Postdoc

Genome Integrity

Education/Working Experience:

2017-            Postdoc   Peking University

2011-2017    Ph.D        Peking University

2007-2011    B.S.          Beijing Normal University


doctor

DR. Tingting Gan

read more

DR. Tingting Gan

Ph.D student

Antibody Diversification

Education:

2017-            Postdoc     Peking University

2016-2021    Ph.D         Peking University

2012-2016    B.S.          Anhui University


doctor

Jianhang Yin

read more

Jianhang Yin

Ph.D student

Genome Editing

Education:

2016-            Ph.D student    Peking University
2011-2015    B.S.                  Peking University


doctor

Weiwei Zhang

read more

Weiwei Zhang

Ph.D student

Genome Editing

Education:

2016-            Ph.D student      Peking University
2009-2013    Bachelor            Xi'an Polytechnic University


doctor

Jinchun Wu

read more

Jinchun Wu

Ph.D student

Genome Integrity


Education:

2017-            Ph.D student    Peking University
2013-2017    B.S.                  Peking University


doctor

Changchang xin

read more

Changchang xin

Ph.D student

Genome Editing

Education:

2018-           Ph.D student    Peking University
2014-2018   B.S.                 Sun Yat-sen University


doctor

Yuefeng Guo

read more

Yuefeng Guo

Ph.D student

Antibody Diversification

Education:

2018             Ph.D student    Peking University
2014-2018    B.S.                  Peking University


doctor

Rusen Lu

read more

Rusen Lu

Ph.D student

Antibody Diversification

Education:

2018-            Ph.D student    Peking University
2014-2018    B.S.                  Peking University


doctor

Yuhong Wang

read more

Yuhong Wang

Ph.D student

Antibody Diversification

Education:

2019-            Ph.D student    Peking University
2015-2019    B.S.                  University of Electronic and Science Technology of China


doctor

Lingyun Kong

read more

Lingyun Kong

Ph.D student

Antibody Diversification

Education:
2020-            Ph.D student      Peking University
2016-2020    B.S.                    Huazhong University of Science and Technology



doctor

Xuhao Liu

read more

Xuhao Liu



无

doctor

Zhengrong Zhangding

read more

Zhengrong Zhangding



无

doctor

Liqiong Ou

read more

Liqiong Ou



无

doctor

Dr. Mengzhu Liu (Alumni)

read more

Dr. Mengzhu Liu (Alumni)

Ph.D student

Genome Editing

Education:

2015-2021    Ph.D    Peking University
2011-2015    B.S.     Lanzhou University


doctor

Dr. Chen Ai (Alumni)

read more

Dr. Chen Ai (Alumni)

Ph.D student

Genome Integrity

Education:

2016-2021    Ph.D        Peking University

2012-2016    B.S.          Huazhong University of Science and Technology


doctor

Dr. Zemin Tan (Alumni)

read more

Dr. Zemin Tan (Alumni)

Postdoc

Immunogenomics

Education:

2009-2015    Ph.D    China Agricultural University
2005-2009    B.S.     China Agricultural University


Working Experience:

2018-2020    PostDoc                 Peking University
2016-2018    R&D supervisor    Beijing kuxun biotechnology co. LTD
2015-2016    R&D supervisor    Beijing norhe zhiyuan biotechnology co. LTD

 

doctor

YOU

read more

YOU



We warmly welcome you to join us and build a positive, innovative, and active young team together.


doctor

Dr. Yang Liu

read more

Dr. Yang Liu

Postdoc

Genome Integrity

Education/Working Experience:

2017-            Postdoc   Peking University

2011-2017    Ph.D        Peking University

2007-2011    B.S.          Beijing Normal University


doctor

DR. Tingting Gan

read more

DR. Tingting Gan

Ph.D student

Antibody Diversification

Education:

2017-            Postdoc     Peking University

2016-2021    Ph.D         Peking University

2012-2016    B.S.          Anhui University


doctor

Jianhang Yin

read more

Jianhang Yin

Ph.D student

Genome Editing

Education:

2016-            Ph.D student    Peking University
2011-2015    B.S.                  Peking University


doctor

Weiwei Zhang

read more

Weiwei Zhang

Ph.D student

Genome Editing

Education:

2016-            Ph.D student      Peking University
2009-2013    Bachelor            Xi'an Polytechnic University


doctor

Jinchun Wu

read more

Jinchun Wu

Ph.D student

Genome Integrity


Education:

2017-            Ph.D student    Peking University
2013-2017    B.S.                  Peking University


doctor

Changchang xin

read more

Changchang xin

Ph.D student

Genome Editing

Education:

2018-           Ph.D student    Peking University
2014-2018   B.S.                 Sun Yat-sen University


doctor

Yuefeng Guo

read more

Yuefeng Guo

Ph.D student

Antibody Diversification

Education:

2018             Ph.D student    Peking University
2014-2018    B.S.                  Peking University


doctor

Rusen Lu

read more

Rusen Lu

Ph.D student

Antibody Diversification

Education:

2018-            Ph.D student    Peking University
2014-2018    B.S.                  Peking University


doctor

Yuhong Wang

read more

Yuhong Wang

Ph.D student

Antibody Diversification

Education:

2019-            Ph.D student    Peking University
2015-2019    B.S.                  University of Electronic and Science Technology of China


doctor

Lingyun Kong

read more

Lingyun Kong

Ph.D student

Antibody Diversification

Education:
2020-            Ph.D student      Peking University
2016-2020    B.S.                    Huazhong University of Science and Technology



doctor

Xuhao Liu

read more

Xuhao Liu



无

doctor

Zhengrong Zhangding

read more

Zhengrong Zhangding



无

doctor

Liqiong Ou

read more

Liqiong Ou



无

doctor

Dr. Mengzhu Liu (Alumni)

read more

Dr. Mengzhu Liu (Alumni)

Ph.D student

Genome Editing

Education:

2015-2021    Ph.D    Peking University
2011-2015    B.S.     Lanzhou University


doctor

Dr. Chen Ai (Alumni)

read more

Dr. Chen Ai (Alumni)

Ph.D student

Genome Integrity

Education:

2016-2021    Ph.D        Peking University

2012-2016    B.S.          Huazhong University of Science and Technology


doctor

Dr. Zemin Tan (Alumni)

read more

Dr. Zemin Tan (Alumni)

Postdoc

Immunogenomics

Education:

2009-2015    Ph.D    China Agricultural University
2005-2009    B.S.     China Agricultural University


Working Experience:

2018-2020    PostDoc                 Peking University
2016-2018    R&D supervisor    Beijing kuxun biotechnology co. LTD
2015-2016    R&D supervisor    Beijing norhe zhiyuan biotechnology co. LTD

 

doctor

YOU

read more

YOU



We warmly welcome you to join us and build a positive, innovative, and active young team together.


Publications

PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair.
 Liu Y#,*, Yin J#, Gan T#, Liu M, Xin C, Zhang W & Hu J* (2022). STAR Protocols 3(1), 101088. doi: 10.1016/j.xpro.2021.101088
Cas9 exo-endonuclease eliminates chromosomal translocations during genome editing.
 Yin J, Lu R, Xin C, Wang Y, Ling X, Li D, Zhang W, Liu M, Xie W, Kong L, Si W, Wei P, Xiao B, Lee HY, Liu T, Hu J. Nat Commun. 2022 Mar 8;13(1):1204. doi: 10.1038/s41467-022-28900-w. PMID: 35260581
RAG2 abolishes RAG1 aggregation to facilitate V(D)J recombination.
 Gan T, Wang Y, Liu Y, Schatz DG, Hu J. Cell Rep. 2021 Oct 12;37(2):109824. doi: 10.1016/j.celrep.2021.109824. PMID: 34644584
Global detection of DNA repair outcomes induced by CRISPR-Cas9.
 Liu M, Zhang W, Xin C, Yin J, Shang Y, Ai C, Li J, Meng FL, Hu J. Nucleic Acids Res. 2021 Sep 7;49(15):8732-8742. doi: 10.1093/nar/gkab686. PMID: 34365511
In-depth assessment of the PAM compatibility and editing activities of Cas9 variants.
 Zhang W, Yin J, Zhang-Ding Z, Xin C, Liu M, Wang Y, Ai C, Hu J. Nucleic Acids Res. 2021 Sep 7;49(15):8785-8795. doi: 10.1093/nar/gkab507. PMID: 34133740
Transcription shapes DNA replication initiation to preserve genome integrity.
 Liu Y, Ai C, Gan T, Wu J, Jiang Y, Liu X, Lu R, Gao N, Li Q, Ji X, Hu J. Genome Biol. 2021 Jun 9;22(1):176. doi: 10.1186/s13059-021-02390-3. PMID: 34108027
Optimizing genome editing strategy by primer-extension-mediated sequencing.
 Yin J, Liu M, Liu Y, Wu J, Gan T, Zhang W, Li Y, Zhou Y, Hu J. Cell Discov. 2019 Mar 26;5:18. doi: 10.1038/s41421-019-0088-8. eCollection 2019. PMID: 30937179
CRISPR/Cas9-mediated targeted chromosome elimination.
 Zuo E, Huo X, Yao X, Hu X, Sun Y, Yin J, He B, Wang X, Shi L, Ping J, Wei Y, Ying W, Wei W, Liu W, Tang C, Li Y, Hu J, Yang H. Genome Biol. 2017 Nov 24;18(1):224. doi: 10.1186/s13059-017-1354-4. PMID: 29178945
Detecting DNA double-stranded breaks in mammalian genomes by linear amplification-mediated high-throughput genome-wide translocation sequencing.
 Hu J, Meyers RM, Dong J, Panchakshari RA, Alt FW, Frock RL. Nat Protoc. 2016 May;11(5):853-71. doi: 10.1038/nprot.2016.043. Epub 2016 Mar 31. PMID: 27031497
Highly sensitive and unbiased approach for elucidating antibody repertoires.
 Lin SG, Ba Z, Du Z, Zhang Y, Hu J, Alt FW. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7846-51. doi: 10.1073/pnas.1608649113. Epub 2016 Jun 27. PMID: 27354528
Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases.
 Frock RL, Hu J, Meyers RM, Ho YJ, Kii E, Alt FW. Nat Biotechnol. 2015 Feb;33(2):179-86. doi: 10.1038/nbt.3101. Epub 2014 Dec 15. PMID: 25503383
Chromosomal loop domains direct the recombination of antigen receptor genes.
 Hu J, Zhang Y, Zhao L, Frock RL, Du Z, Meyers RM, Meng FL, Schatz DG, Alt FW. Cell. 2015 Nov 5;163(4):947-59. doi: 10.1016/j.cell.2015.10.016. Epub 2015 Oct 22. PMID: 26593423
Mechanisms that can promote peripheral B-cell lymphoma in ATM-deficient mice.
 Tepsuporn S, Hu J, Gostissa M, Alt FW. Cancer Immunol Res. 2014 Sep;2(9):857-66. doi: 10.1158/2326-6066.CIR-14-0090. Epub 2014 Jun 9. PMID: 24913718
Developmental propagation of V(D)J recombination-associated DNA breaks and translocations in mature B cells via dicentric chromosomes.
 Hu J, Tepsuporn S, Meyers RM, Gostissa M, Alt FW. Proc Natl Acad Sci U S A. 2014 Jul 15;111(28):10269-74. doi: 10.1073/pnas.1410112111. Epub 2014 Jun 30. PMID: 24982162
The intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing.
 Hu J, Sun L, Shen F, Chen Y, Hua Y, Liu Y, Zhang M, Hu Y, Wang Q, Xu W, Sun F, Ji J, Murray JM, Carr AM, Kong D. Cell. 2012 Jun 8;149(6):1221-32. doi: 10.1016/j.cell.2012.04.030. PMID: 22682245
Search
Years
Keywords
antibody antigen B-cell crispr double-stranded breaks replication translocation